P-707 Evaluating DualStim/PGTA in poor prognosis patients: Comparison of double ICSI-PGTA vs. oocyte cryopreservation plus single PGTA in luteal phase through laboratory, genetic and clinical results.

Abstract Study question Which strategy is best for poor-prognosis patients: Two ICSI-PGTA cycles fresh oocytes (follicular plus luteal phase) or one cycle with vitrified and oocytes?" Summary answer Laboratory outcomes, ploidy rates clinical results after euploid blastocyst transfer are similar between the two DualStim/PGTA strategies. What known already Luteal phase ovarian stimulation (LPOS) follicular (FPOS) an effective to obtain more in a single reduce time-to-pregnancy. The main goal of controlled PGTA patients sufficient number have at least blastocyst. standard treatment achieving this DuoStim oocyte cryopreservation. However, method may decrease competence older patients. To address this, we propose comparing outcomes consecutive our protocol patients, as alternative approach. design, size, duration This multicentric retrospective study evaluated 91 (≤ 5 antral follicle count) using DualStim approach (182 pick-ups) same menstrual cycle, from January October 2022. Fifty-three (group 1; 746 retrieved) underwent egg retrievals, ICSI, biopsy, FPOS LPOS, while 38 2; 743 vitrification FPOS, followed by retrieval, warming, biopsy. Participants/materials, setting, methods We compared various parameters including number, maturity, fertilization rates, quality, biopsy genetic groups both retrievals. Additionally, embryo cryotransfer were also compared. Oocytes microinjected, cultured Geri® time-lapse incubators, on day 5/6. Statistical analysis was conducted R software (v.4.2.0), maternal age confounding factor. Results considered significant p-value less than 0.05. Main role chance Patient found be significantly different (40.5±2.2 group-1 vs 38.5±2.7 group-2; p < 0.001) used factor statistical comparisons. No differences terms retrieved per patient (14±7.1 13.6±5.8), maturity rate (79.4 % 77.9%), (75.3 74.6%), day-5 (54.8% 51.7%). difference observed percentage blastocysts biopsied group (50.2% 43.0% = 0.023). After analysis, euploidy lower group-2 although not statistically (19.8% 32.6%, respectively, 0.007/p=0.309 adjusting age), led average embryos (1.1±1.3 1.3±1.4 group-2). Aneuploidy (73.9% 62%) mosaicism (6.3% 5.4%) comparable groups. phases each laboratory results. Preliminary showed pregnancy 12 pregnancies achieved 1 23 transfers (52.2%), 14/20 2 (70.0%) no Limitations, reasons caution further validation through prospective recommended. due bias prescription treatment, corrected. Wider implications findings Cryopreserving obtained warming them subsequent LPOS does negatively impact quality final cycle. should it can workload IVF minimize costs Trial registration none